fruitПлодоводствоFruit Growing0134-9759Институт плодоводстваfruit-30Research ArticleПЛОДОВОДСТВО И ЯГОДОВОДСТВО В БЕЛАРУСИ И ЗА РУБЕЖОМFRUIT GROWING AND BERRY GROWING IN BELARUS AND ABROADРаспространение вирусных и вирусоподобных патогенов семечковых культур в УкраинеDistribution of virus and virus-like pathogens of pome fruits in UkraineУдовиченкоЕ. Н.UdovychenkoE. N.

ул. Садовая, 23, с. Новоселки, Киево-Святошинский р-н, Киевская обл., 03027, Украина

k_udovychenko@ukr.netРябаИ. А.RiabaI. A.

ул. Садовая, 23, с. Новоселки, Киево-Святошинский р-н, Киевская обл., 03027, Украина

ПавлюкЛ. В.PavliukL. V.

ул. Садовая, 23, с. Новоселки, Киево-Святошинский р-н, Киевская обл., 03027, Украина

ГрыныкИ. В.HrynykI. V.

ул. Садовая, 23, с. Новоселки, Киево-Святошинский р-н, Киевская обл., 03027, Украина

Институт садоводства НААН УкраиныUkraine2020220320223212731Copyright © Удовиченко Е.Н., Ряба И.А., Павлюк Л.В., Грынык И.В., 20222022Удовиченко Е.Н., Ряба И.А., Павлюк Л.В., Грынык И.В.Udovychenko E.N., Riaba I.A., Pavliuk L.V., Hrynyk I.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://fruit.belal.by/jour/article/view/30

Проведено исследование распространенности в Украине вирусов, вироидов и фитоплазм семечковых культур методами ELISA и RT-PCR. Во всех исследованных областях на сортах и подвоях яблони и груши выявлены вирусы ACLSV, ASGV, ASPV, ApMV. В Киевской, Закарпатской и Донецкой областях диагностировали зараженность фитоплазмами. Установлено отсутствие в исследованных образцах ToRSV, TRSV, ASSVd, ADFVd и PBCVd. В результате тестирования отобраны растения-кандидаты в безвирусные клоны 32 сортов и 3 подвоев яблони, 13 сортов и 4 подвоев груши.

A study of the occurence in Ukraine of viruses, viroids and phytoplasmas of pome fruits by ELISA and RT-PCR methods was carried out. In all studied regions, ACLSV, ASGV, ASPV, ApMV viruses were detected on cultivars and roostocks of apple and pear. In Kyiv, Zakarpattya and Donetsk regions phytoplasma infection was diagnosed. The absence of ToRSV, TRSV, ASSVd, ADFVd, and PBCVd in the studied samples was determined. As a result of testing, candidate nuclear-stock plants of 32 apple cultivars and 3 apple rootstocks, 13 pear cultivars and 4 pear rootstocks were selected.

яблонягрушавирусывироидыфитоплазмыУкраинаapplepearvirusesviroidsphytoplasmaUkraineReferencesГосподарик, А. В. Діагностика вірусів плодових культур в умовах України / А. В. Господарик, К. М. Удовиченко, В. П. Поліщук // Наук. зап. НаУКМА. Сер. Біологія та екологія. – 2005. – Т. 43. – С. 51–53.Господарик, А. В. Діагностика вірусів плодових культур в умовах України / А. В. Господарик, К. М. Удовиченко, В. П. Поліщук // Наук. зап. НаУКМА. Сер. Біологія та екологія. – 2005. – Т. 43. – С. 51–53.Barba, M. Control of pome and stone fruit virus diseases / M. Barba, V. Ilardi, G. Pasquini // Adv. Virus Res. – 2015. – № 91. – Р. 47–83.Barba, M. Control of pome and stone fruit virus diseases / M. Barba, V. Ilardi, G. Pasquini // Adv. Virus Res. – 2015. – № 91. – Р. 47–83.Deng, S. Amplification of 16S rRNA genes from culturable and nonculturable mollicutes / S. Deng, D. Hiruki // Journal of Microbiological Methods. – 1991. – Vol. 14. – P. 53–61.Deng, S. Amplification of 16S rRNA genes from culturable and nonculturable mollicutes / S. Deng, D. Hiruki // Journal of Microbiological Methods. – 1991. – Vol. 14. – P. 53–61.Other Apscaviroids Infecting Pome Fruit Trees / F. Di Serio [et al.]. In: Hadidi A, Flores R, Randles J, Palukaitis P (eds.). Viroids and Satellites. Academic press. – London UK. – 2017b. – Р. 229–241.Other Apscaviroids Infecting Pome Fruit Trees / F. Di Serio [et al.]. In: Hadidi A, Flores R, Randles J, Palukaitis P (eds.). Viroids and Satellites. Academic press. – London UK. – 2017b. – Р. 229–241.EPPO Standards. PM 4/27(1) [Electronic resource] Pathogen-tested material of Malus, Pyrus and Cydonia. – Mode of access: http://archives.eppo.int/EPPOStandards/certification.htm. – Date of access: 30.04.2020.EPPO Standards. PM 4/27(1) [Electronic resource] Pathogen-tested material of Malus, Pyrus and Cydonia. – Mode of access: http://archives.eppo.int/EPPOStandards/certification.htm. – Date of access: 30.04.2020.ISPM 27. Annex 12. Phytoplasmas (2016). Rome, IPPC, FAO. – Mode of access: https://www.ippc.int/static/media/files/publication/en/2016/04/DP_12_2016_En_2016-04-14.pdf. – Date of access: 30.04.2020.ISPM 27. Annex 12. Phytoplasmas (2016). Rome, IPPC, FAO. – Mode of access: https://www.ippc.int/static/media/files/publication/en/2016/04/DP_12_2016_En_2016-04-14.pdf. – Date of access: 30.04.2020.Detection of multiple phytoplasmas in perennial fruit trees with decline symptoms in Italy / I. M. Lee [et al.] // Phytopathology. – 1995. – Vol. 85. – P. 728–735.Detection of multiple phytoplasmas in perennial fruit trees with decline symptoms in Italy / I. M. Lee [et al.] // Phytopathology. – 1995. – Vol. 85. – P. 728–735.Loreti, S. Identification and characterization of an Italian isolate of Pear blister canker viroid / S. Loreti, F. Fagioli, M. Barba // Journal of Phytopathology. – 1997. – Vol. 145. – P. 541–544.Loreti, S. Identification and characterization of an Italian isolate of Pear blister canker viroid / S. Loreti, F. Fagioli, M. Barba // Journal of Phytopathology. – 1997. – Vol. 145. – P. 541–544.Menzel, W. Detection of four apple viruses by multiplex RT-PCR assays with coamplification of plant mRNA as internal control / W. Menzel, W. Jelkmann, E. Maiss // J Virol Methods. – 2002. – № 99. – P. 81–92.Menzel, W. Detection of four apple viruses by multiplex RT-PCR assays with coamplification of plant mRNA as internal control / W. Menzel, W. Jelkmann, E. Maiss // J Virol Methods. – 2002. – № 99. – P. 81–92.Moini, A. A. Identification of Tomato ringspot virus (ToRSV) on apple in Iran / A. A. Moini // Australasian Plant Disease Notes. – 2010. – Vol. 5. – P. 105–106.Moini, A. A. Identification of Tomato ringspot virus (ToRSV) on apple in Iran / A. A. Moini // Australasian Plant Disease Notes. – 2010. – Vol. 5. – P. 105–106.Virological assessment of stock planting material of apple and raspberry cultivars / J. Stankienė [et al.] // Žemdirbystė Agriculture. – 2012. – Vol. 99, № 1. – P. 93–98.Virological assessment of stock planting material of apple and raspberry cultivars / J. Stankienė [et al.] // Žemdirbystė Agriculture. – 2012. – Vol. 99, № 1. – P. 93–98.

The authors declare that there are no conflicts of interest present.