fruitПлодоводствоFruit Growing0134-9759Институт плодоводстваfruit-31Research ArticleПЛОДОВОДСТВО И ЯГОДОВОДСТВО В БЕЛАРУСИ И ЗА РУБЕЖОМFRUIT GROWING AND BERRY GROWING IN BELARUS AND ABROADСравнительная оценка эффективности лабораторных методов (ПЦР, DAS-ELISA) диагностики фитоплазмы яблони в БеларусиComparative evaluation of the efficiency of laboratory methods of diagnostics (PCR, DAS-ELISA) of phytoplasma diseases of apple in BelarusКолбановаЕ. В.KolbanovaE. V.

ул. Ковалева, 2, аг. Самохваловичи, Минский район, 223013, Беларусь

kolbanova@tut.byБожидайТ. Н.BozhidayT. N.

ул. Ковалева, 2, аг. Самохваловичи, Минский район, 223013, Беларусь

КухарчикН. В.KukharchykN. V.

ул. Ковалева, 2, аг. Самохваловичи, Минский район, 223013, Беларусь

РУП «Институт плодоводства»Belarus2020220320223213237Copyright © Колбанова Е.В., Божидай Т.Н., Кухарчик Н.В., 20222022Колбанова Е.В., Божидай Т.Н., Кухарчик Н.В.Kolbanova E.V., Bozhiday T.N., Kukharchyk N.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://fruit.belal.by/jour/article/view/31

Методом ПЦР в реальном времени с праймерами Phyto-F/Phyto-R и зондом Phyto-P фитоплазма яблони была обнаружена у 17 образцов из 25 тестируемых, все 8 деревьев, имеющие визуальные симптомы («ведьмины метлы»), показали положительный результат.

Использование DAS-ELISA-теста в середине лета (июль) для диагностики фитоплазмы яблони дало 88,2 % ложноотрицательных результатов: из 17 зараженных образцов, выявленных методом ПЦР, только 2 образца оказались положительными. Использование DAS-ELISA-теста осенью (сентябрь) также оказалось малорезультативным – 70,6 % ложноотрицательных результата (из 17 зараженных образцов, выявленных методом ПЦР, только 5 образцов показали положительный результат).

Метод ПЦР в реальном времени по сравнению с DAS-ELISA-тестом является более эффективным методом для диагностики фитоплазмы яблони.

Using real-time PCR with primer pair Phyto-F/Phyto-R and probe Phyto-P, phytoplasma diseases of apple was detected in 17 samples of 25 tested, all the 8 trees with visual symptoms («witches’ broom») showed a positive result. 88.2 % of false-negative results were obtained using the DAS-ELISA test in mid-summer (July) for diagnosis of phytoplasma diseases of apple (only 2 samples were positive from 17 infected samples detected by PCR). 70.6 % of falsenegative results (only 5 samples were positive from 17 infected samples detected by PCR) were obtained using the DASELISA test in the fall (September). Real-time PCR compared with the DAS-ELISA test is a more effective method for the diagnosis of phytoplasma diseases of apple.

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The authors declare that there are no conflicts of interest present.