fruitПлодоводствоFruit Growing0134-9759Институт плодоводстваfruit-358Research ArticleПЛОДОВОДСТВО И ЯГОДОВОДСТВО В БЕЛАРУСИFRUIT AND SMALL FRUIT GROWING IN ВELARUSАнализ зараженности вирусом Шарки сливы сортов сливы, алычи и вишни в БеларусиAnalysis of infection rate by Plum pox virus of plum, cherry plum and sour cherry cultivars in BelarusКухарчикН. В.KukharchikN. V.

ул. Ковалева, 2, аг. Самохваловичи, Минский район, 223013, Беларусь

Kychnataly@rambler.ruКастрицкаяМ. С.KastritskayaM. S.

ул. Ковалева, 2, аг. Самохваловичи, Минский район, 223013, Беларусь

СоловейО. В.SolovejO. V.

ул. Ковалева, 2, аг. Самохваловичи, Минский район, 223013, Беларусь

РУП «Институт плодоводства»Belarus201412052022261136142Copyright © Кухарчик Н.В., Кастрицкая М.С., Соловей О.В., 20222022Кухарчик Н.В., Кастрицкая М.С., Соловей О.В.Kukharchik N.V., Kastritskaya M.S., Solovej O.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://fruit.belal.by/jour/article/view/358

Проведен анализ зараженности сортов сливы, алычи, вишни и черешни белорусского сортимента вирусом Шарки сливы (Plum pox virus). Предварительные исследования проведены методом маршрутных обследований, основные – иммуноферментным анализом с использованием поликлональных (РАb) и универсальных моноклональных (PPV-Un MAbs) антител к вирусу Шарки, а также методом IC-RT-PCR с праймерами PPV-P1/PPV-P2. Мониторинг вируса Шарки сливы в маточно-черенковых насаждениях, маточниках клоновых подвоев и промышленных садах, проводящийся в течение 2000–2013 гг., позволил оценить поражаемость конкретных сортов и форм подвоев вирусом на естественном инфекционном фоне (59 генотипов).

Вирус Шарки выявлен у сортов сливы Награда неманская, Витебская поздняя, Empress, Президент; сортов алычи Комета, Найдена, Ветразь, сорта вишни Вянок, сорта черешни Ипуть, подвоев ОД-2-3, ВВА-1, 140-2, Л-2 и семенных подвоев. Наибольшее количество пораженных Plum pox virus растений отмечено у сорта сливы Награда неманская и сорта алычи Комета.

The analysis of infection rate of plum, cherry plum, sour cherry and sweet cherry cultivars of the Belarusian assortment by Sharka plum virus (Plum pox virus) was carried out. Preliminary investigations were made by the method of routing inspections. Basic researches were carried out with the help of enzyme immunoassay using polyclonal antibodies (РАb) and universal monoclonal ones (PPV-UnMAbs) to the plum pox virus, as well as with the help of IC-RT-PCR method with primers PPV-P1/PPV-P2. Monitoring of plum pox virus in mother and cutting plantations, in mother plantations of clonal stocks and in industrial orchards was made within 2000-2013, which allowed estimating the infection rate of certain cultivars and stock forms by the virus on a natural infectious background (59 genotypes).

Plum pox virus has been revealed at numerous fruit crops cultivars. Among them are plum cultivars Nagrada nemanskaya, Vitebskaya pozdnyaya, Empress and Prezident; cherry plum ones such as Kometa, Naidzena and Vetraz‘; cherry cultivar Vyanok; sweet cherry one Iputs; stocks OD-2-3, VVA-1, 140-2, L-2 and seed stocks. The greatest number of plants affected by Plum pox virus was at the plum cultivar Nagrada nemanskaya and cherry plum cultivar Kometa.

Plum pox virusсливадиагностикаиммуноферментный анализПЦРБеларусьPlum pox virusplumdiagnosisenzyme immunoassayPCRBelarusReferencesQuarantine Pests for Europe. Data sheets on Quarantine Pests for the European Communities and for the European and Mediterranean Plant Protection Oganization. Prepead by CABI and EPPO for EC [Electronic resource]. – 1992. – Mode of access: https://www.eppo.int/.../virus/Plum_pox_virus/PPV000_ds.pdf. – Date of access: 20.02.2014.Quarantine Pests for Europe. Data sheets on Quarantine Pests for the European Communities and for the European and Mediterranean Plant Protection Oganization. Prepead by CABI and EPPO for EC [Electronic resource]. – 1992. – Mode of access: https://www.eppo.int/.../virus/Plum_pox_virus/PPV000_ds.pdf. – Date of access: 20.02.2014.Quarantine Pests for Europe. Date sheets on Quarantine Peats for European Communities and for the European and Mediterranean Plant Protaction Organization. Prepead by CABI and EPPO for EC [Electronic resource]. – 1997. – Mode of access: www.eppo.int/.../virus/Plum_pox_virus/pm7-32(1)%20PPV000%20web.pdf. – Date of access: 12.02.2014.Quarantine Pests for Europe. Date sheets on Quarantine Peats for European Communities and for the European and Mediterranean Plant Protaction Organization. Prepead by CABI and EPPO for EC [Electronic resource]. – 1997. – Mode of access: www.eppo.int/.../virus/Plum_pox_virus/pm7-32(1)%20PPV000%20web.pdf. – Date of access: 12.02.2014.Plum pox potyvirus disease of stone fruits [Electronic resource]. – 1997. – Mode of access: https://www.apsnet.org/publications/apsnetfeatures/Pages/PlumPoxPotyvirus.aspx. – Date of access: 10.02.2014.Plum pox potyvirus disease of stone fruits [Electronic resource]. – 1997. – Mode of access: https://www.apsnet.org/publications/apsnetfeatures/Pages/PlumPoxPotyvirus.aspx. – Date of access: 10.02.2014.Nemeth, M. History and importance of plum pox in stone-fruit production / M. Nemeth // EPPO Bull. – 1994. – N 24. – P. 525-526.Nemeth, M. History and importance of plum pox in stone-fruit production / M. Nemeth // EPPO Bull. – 1994. – N 24. – P. 525-526.Вердеревская, Т.Д. Вирусные и микоплазменные заболевания плодовых культур и винограда / Т.Д. Вердеревская, В.Г. Маринеску. – Кишинев, Штиинца, 1985. – 311 с.Вердеревская, Т.Д. Вирусные и микоплазменные заболевания плодовых культур и винограда / Т.Д. Вердеревская, В.Г. Маринеску. – Кишинев, Штиинца, 1985. – 311 с.Myrta, A. Production of two monoclonal antibodies specific to cherry strain plum pox virus (PPV-C) / A. Myrta [et al.] // J. Plant Pathol. – 2000. – N 82 (suppl. 2). – P. 95-103.Myrta, A. Production of two monoclonal antibodies specific to cherry strain plum pox virus (PPV-C) / A. Myrta [et al.] // J. Plant Pathol. – 2000. – N 82 (suppl. 2). – P. 95-103.Myrta, A. Production of a monoclonal antibody specific to the EL-Amar strain of plum pox virus / A. Myrta [et al.] // ActaVirol. – 1998. – N 42. – P. 248-250.Myrta, A. Production of a monoclonal antibody specific to the EL-Amar strain of plum pox virus / A. Myrta [et al.] // ActaVirol. – 1998. – N 42. – P. 248-250.Nemchinov, L. Characterization of the sour cherry of plum pox virus / L. Nemchinov, A. Hadidi // Phytopathology. – 1996. – N 86. – P. 575-580.Nemchinov, L. Characterization of the sour cherry of plum pox virus / L. Nemchinov, A. Hadidi // Phytopathology. – 1996. – N 86. – P. 575-580.Nemchinov, L. Sour cherry strain of plum pox potyvirus (PPV): molecular and serological evidence for a new subgroup of PPV strains / L. Nemchinov [et al.] // Phytopathology. – 1996. – N 86. – P. 1215-1221.Nemchinov, L. Sour cherry strain of plum pox potyvirus (PPV): molecular and serological evidence for a new subgroup of PPV strains / L. Nemchinov [et al.] // Phytopathology. – 1996. – N 86. – P. 1215-1221.EPPO/OEPP. SMT Project SMT-4-CT98-2252 (Протокол диагностики карантинного организма - PPV) // Bulletin OEPP/EPPO. – 2002. – N 34. – P. 155-157.EPPO/OEPP. SMT Project SMT-4-CT98-2252 (Протокол диагностики карантинного организма - PPV) // Bulletin OEPP/EPPO. – 2002. – N 34. – P. 155-157.Salavei, A. Detection of plum pox virus in regions of Belarus / A. Salavei [et al.] // 22nd ―International Conference on Virus and Other Trandmissible Diseases of Fruit Crops‖, Rome, 5-8 Jun 2012 / ICVF; Scientific Committee: W. Jelkmann [et al.]. – Rome, 2012. – Р. 152.Salavei, A. Detection of plum pox virus in regions of Belarus / A. Salavei [et al.] // 22nd ―International Conference on Virus and Other Trandmissible Diseases of Fruit Crops‖, Rome, 5-8 Jun 2012 / ICVF; Scientific Committee: W. Jelkmann [et al.]. – Rome, 2012. – Р. 152.Malinowski, T. Partial characterisation of biological properties of PPV-C isolates found in Belarus and establishment of in vitro cultures of infected L2 and OWP-6 rootstocks / T. Malinowski [et al.] // 22nd ―International Conference on Virus and Other Trandmissible Diseases of Fruit Crops‖, Rome, 5-8 Jun 2012 / ICVF; Scientific Committee: W. Jelkmann [et al.]. – Rome, 2012. – P. 149.Malinowski, T. Partial characterisation of biological properties of PPV-C isolates found in Belarus and establishment of in vitro cultures of infected L2 and OWP-6 rootstocks / T. Malinowski [et al.] // 22nd ―International Conference on Virus and Other Trandmissible Diseases of Fruit Crops‖, Rome, 5-8 Jun 2012 / ICVF; Scientific Committee: W. Jelkmann [et al.]. – Rome, 2012. – P. 149.Salavei, A.V. Sanitary status of Sharka on Stone fruit trees in Belarus / A.V. Salavei, N.V. Kukharchyk, T. Malinovski // International symposium on Plum pox virus, Sofia, September 5-9, 2010, Bulgaria. – Sofia, 2010. – P. 39.Salavei, A.V. Sanitary status of Sharka on Stone fruit trees in Belarus / A.V. Salavei, N.V. Kukharchyk, T. Malinovski // International symposium on Plum pox virus, Sofia, September 5-9, 2010, Bulgaria. – Sofia, 2010. – P. 39.

The authors declare that there are no conflicts of interest present.