fruitПлодоводствоFruit Growing0134-9759Институт плодоводства10.47612/0134-9759-2021-33-40-47fruit-8Research ArticleПЛОДОВОДСТВО И ЯГОДОВОДСТВО В БЕЛАРУСИ И ЗА РУБЕЖОМFRUIT GROWING AND BERRY GROWING IN BELARUS AND ABROADВстречаемость фитоплазмы в насаждениях яблони в БеларусиFrequency occurrence of phytoplasma in apple plantations in BelarusКолбановаЕ. В.KolbanovaE. V.

ул. Ковалёва, 2, аг. Самохваловичи, Минский район, 223013

kolbanova@tut.byБожидайТ. Н.BozhidaiT. N.

ул. Ковалёва, 2, аг. Самохваловичи, Минский район, 223013

КухарчикН. В.KukharchikN. V.

ул. Ковалёва, 2, аг. Самохваловичи, Минский район, 223013

СидоренкоТ. Н.SidorenkoT. N.

аг. Довск, Рогачевский район, Гомельская область, 247261

goshos@mail.gomel.byРУП «Институт плодоводства»BelarusРУП «Гомельская областная сельскохозяйственная опытная станция» НАН БеларусиBelarus2021210320223304047Copyright © Колбанова Е.В., Божидай Т.Н., Кухарчик Н.В., Сидоренко Т.Н., 20222022Колбанова Е.В., Божидай Т.Н., Кухарчик Н.В., Сидоренко Т.Н.Kolbanova E.V., Bozhidai T.N., Kukharchik N.V., Sidorenko T.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://fruit.belal.by/jour/article/view/8

Пролиферация яблони (Apple proliferation) – заболевание растений, вызываемое фитоплазмой Candidatus Phytoplasma mali, принадлежащей к группе пролиферации яблони (Apple proliferation (AP) group), подгруппе 16SrX-A. Поражение растений фитоплазмой приводит к изменению характера ветвления побегов, измельчению плодов, а часто и к гибели растений, нанося ущерб сельскохозяйственному производству.

С целью оценки встречаемости фитоплазмы в насаждениях яблони в Беларуси было проведено обследование садов Минской, Брестской, Гомельской, Могилевской областей. Отобрано (с симптоматичных или угнетенных деревьев) и протестировано 453 образца (60 генотипов). По итогам обследования и тестирования методом ПЦР в реальном времени с праймерами Phyto-F/Phyto-R и зондом Phyto-P выявлено наличие фитоплазмы в насаждениях яблони в Гомельской (61 зараженный образец из 177 протестированных), Минской (18 из 112) и Могилевской областях (48 из 67) и отсутствие патогена в насаждениях Брестской области.

Apple-tree proliferation is a plant disease caused by phytoplasma Candidatus Phytoplasma mali which belongs to the Аpple proliferation (AP) group, subgroup 16SrX-A. The damage of plants by phytoplasm leads to changes in the nature of branching of shoots, crushing of fruits and often to the death of plants, causing damage to agricultural production.

In order to assess the frequency occurrence of phytoplasm in apple-tree plantations in Belarus, a survey was carried out of the examination of orchards of Minsk, Brest, Gomel and Mogilev regions. There were selected (from symptomatic or damaged trees) and tested 453 samples (60 genotypes). Based on the results of examination and testing by real-time PCR with Phyto-F/Phyto-R primers and Phyto-P probe, the presence of phytoplasm in apple-tree plantations in Gomel (61 infected samples out of 177 tested), Minsk (18 out of 112) and Mogilev regions was revealed (48 out of 67) and the absence of a pathogen in the plantations of the Brest region.

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The authors declare that there are no conflicts of interest present.