Preview

Fruit Growing

Advanced search

Frequency occurrence of phytoplasma in apple plantations in Belarus

https://doi.org/10.47612/0134-9759-2021-33-40-47

Abstract

Apple-tree proliferation is a plant disease caused by phytoplasma Candidatus Phytoplasma mali which belongs to the Аpple proliferation (AP) group, subgroup 16SrX-A. The damage of plants by phytoplasm leads to changes in the nature of branching of shoots, crushing of fruits and often to the death of plants, causing damage to agricultural production.

In order to assess the frequency occurrence of phytoplasm in apple-tree plantations in Belarus, a survey was carried out of the examination of orchards of Minsk, Brest, Gomel and Mogilev regions. There were selected (from symptomatic or damaged trees) and tested 453 samples (60 genotypes). Based on the results of examination and testing by real-time PCR with Phyto-F/Phyto-R primers and Phyto-P probe, the presence of phytoplasm in apple-tree plantations in Gomel (61 infected samples out of 177 tested), Minsk (18 out of 112) and Mogilev regions was revealed (48 out of 67) and the absence of a pathogen in the plantations of the Brest region.

About the Authors

E. V. Kolbanova
РУП «Институт плодоводства»
Belarus


T. N. Bozhidai
РУП «Институт плодоводства»
Belarus


N. V. Kukharchik
РУП «Институт плодоводства»
Belarus


T. N. Sidorenko
РУП «Гомельская областная сельскохозяйственная опытная станция» НАН Беларуси
Belarus


References

1. Seemuller, E. ‘Candidatus Phytoplasma mali’, ‘Candidatus Phytoplasma pyri’ and ‘Candidatus Phytoplasma prunorum’, the causal agents of apple proliferation, pear decline and European stone fruit yellows, respectively / E. Seemuller, B. Schneider // Int. J. Syst. Evol. Microbiol. – 2004. – Vol. 54. – P. 1217–1226.

2. Revised classification scheme of phytoplasmas based on RFLP analyses of 16S rRNA and ribosomal protein gene sequences / I.-М. Lee [et al.] // Int. J. Syst. Bacteriol. – 1998. – Vol. 48, № 4. – P. 1153–1169.

3. PM 7/62 (2) ‘Candidatus phytoplasma mali’, ‘Ca. P. pyri’ and ‘Ca. P. prunorum’ // Bull. OEPP/EPPO. – 2017. – Vol. 47. – P. 146–163.

4. Bertaccini, A. Outlook on relevant phytoplasma diseases in Europe [Electronic resource] / A. Bertaccini, B. Duduk // New perspectives in phytoplasma disease management / COST action FA0807 Workshop. – Barcelona, Spain, 2013. – P. 11– 16. – Mode of access: http://costphytoplasma.ipwgnet.org/PDF%20files/BOOK%20COST%20BCN%202013%20080313web. pdf. – Date of access: 02.03.2019.

5. Bertaccini, A. Phytoplasma and phytoplasma diseases: a review of recent research / А. Bertaccini, B. Duduk // Phytopath. Mediterranea. – 2009. – Vol. 48, № 3. – P. 355–378.

6. Marzachi, C. Molecular diagnosis of phytoplasmas / C. Marzachi // Arab J. Plant Prot. – 2006. – Vol. 24, № 2. – P. 139–142.

7. Berges, R. Range of phytoplasma concentration in various hosts as determined by competitive polymerase chain reaction / R. Berges, M. Rott, E. Seemuller // Phytopath. – 2000. – Vol. 90. – P. 1145–1152.

8. Constable, F. E. Seasonal distribution of phytoplasmas in Australian grapevines / F. E. Constable, K. S. Gibb, R. H. Symons // Plant Pathol. – 2003. – Vol. 52. – P. 267–276.

9. Seasonal detection of pear decline phytoplasma by nested-PCR in different pear cultivars / M. Garcia-Chapa [et al.] // Plant Pathol. – 2003. – Vol. 52. – P. 513–520.

10. Spatiotemporal distribution of flavescence doree phytoplasma in grapevine / N. Prezelj [et al.] // Plant Pathol. – 2012. – Vol. 62. – P. 760–766.

11. Distribution of phytoplasmas in infected plants as revealed by real-time PCR and bioimaging / N. M. Christensen [et al.] // Mol. Plant – Microbe Interact. – 2004. – Vol. 17, № 11. – P. 1175–1184.


Review

For citations:


Kolbanova E.V., Bozhidai T.N., Kukharchik N.V., Sidorenko T.N. Frequency occurrence of phytoplasma in apple plantations in Belarus. Fruit Growing. 2021;33:40-47. (In Russ.) https://doi.org/10.47612/0134-9759-2021-33-40-47

Views: 181


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.


ISSN 0134-9759 (Print)