Comparative evaluation of the efficiency of laboratory methods of diagnostics (PCR, DAS-ELISA) of phytoplasma diseases of apple in Belarus
Abstract
Using real-time PCR with primer pair Phyto-F/Phyto-R and probe Phyto-P, phytoplasma diseases of apple was detected in 17 samples of 25 tested, all the 8 trees with visual symptoms («witches’ broom») showed a positive result. 88.2 % of false-negative results were obtained using the DAS-ELISA test in mid-summer (July) for diagnosis of phytoplasma diseases of apple (only 2 samples were positive from 17 infected samples detected by PCR). 70.6 % of falsenegative results (only 5 samples were positive from 17 infected samples detected by PCR) were obtained using the DASELISA test in the fall (September). Real-time PCR compared with the DAS-ELISA test is a more effective method for the diagnosis of phytoplasma diseases of apple.
About the Authors
E. V. Kolbanova
РУП «Институт плодоводства»
Belarus
Belarus
T. N. Bozhiday
РУП «Институт плодоводства»
Belarus
Belarus
N. V. Kukharchyk
РУП «Институт плодоводства»
Belarus
Belarus
References
1. Maejima, K. Exploring the phytoplasmas, plant pathogenic bacteria / K. Maejima, K. Oshima, Sh. Namba // Journal of General Plant Pathology. – 2014. – Vol. 80 – P. 210–221.
2. Eben, A. Innovative vector control [Electronic resource] / A. Eben, J. Gross // New perspectives in phytoplasma disease management / COST action FA0807 Workshop. – Barcelona, Spain, 2013. – P. 38–40. – Mode of access: http://costphytoplasma.
3. ipwgnet.org/PDF%20files/BOOK%20COST%20BCN%202013%20080313web.pdf. – Date of access: 02.03.2019.
4. Bertaccini, A. Phytoplasma and phytoplasma diseases: a review of recent research / А. Bertaccini, B. Duduk // Phytopathologia Mediterranea. – 2009. – Vol. 48, № 3. – P. 355–378.
5. Marzachi, C. Molecular diagnosis of phytoplasmas / C. Marzachi // Arab Journal of Plant Protection. – 2006. – Vol. 24, № 2. – P. 139–142.
6. Berges, R. Range of phytoplasma concentration in various hosts as determined by competitive polymerase chain reaction / R. Berges, M. Rott, E. Seemuller // Phytopathology. – 2000. – Vol. 90. – P. 1145–1152.
7. Constable, F. E. Seasonal distribution of phytoplasmas in Australian grapevines / F.E. Constable, K. S. Gibb, R. H. Symons // Plant Pathology. – 2003. – Vol. 52. – P. 267–276.
8. Seasonal detection of pear decline phytoplasma by nested-PCR in different pear cultivars / M. Garcia-Chapa [et al.] // Plant Pathology. – 2003. – Vol. 52. – P. 513–520.
9. Spatiotemporal distribution of flavescence doree phytoplasma in grapevine / N. Prezelj [et al.] // Plant Pathology. – 2012. – Vol. 62. – P. 760–766.
10. Detection of apple proliferation by ELISA and PCR in growing and dormant apple trees / J. Brzin [et al.] // Plant Diseases and Protection. – 2003. – Vol. 110. – P. 476–483.
11. Spreading and characterization of ‘Candidatus Phytoplasma mali’ subtypes in different growing areas / M. Martini [et al.] // Petria. – 2005. – Vol. 15. – P. 105–107.
12. Distribution of phytoplasmas in infected plants as revealed by real-time PCR and bioimaging / N. M. Christensen [et al.] // Molecular Plant – Microbe Interactions. – 2004. – Vol. 17, № 11. – P. 1175–1184.
Review
For citations:
Kolbanova E.V., Bozhiday T.N., Kukharchyk N.V. Comparative evaluation of the efficiency of laboratory methods of diagnostics (PCR, DAS-ELISA) of phytoplasma diseases of apple in Belarus. Fruit Growing. 2020;32(1):32-37. (In Russ.)
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