Preview

Плодоводство

Расширенный поиск

Скрининг устойчивости местных сортов яблони Азербайджана к патогенам Venturia inaequalis (Cooke.) Wint. и Podosphaera leucotricha Salm. с использованием молекулярных маркеров

Аннотация

В данном исследовании с помощью молекулярных подходов была оценена устойчивость к патогену Venturia inaequalis (Cooke.) Wint. некоторых местных сортов яблони, произрастающих на территории Азербайджана. Использовано 27 молекулярных маркеров генов устойчивости к парше и три маркера устойчивости к мучнистой росе. В качестве объектов использовали двадцать коллекционных сортов яблони Института JKI, Дрезден, Германия, устойчивых к парше, и семь местных сортов яблони, произрастающих в Азербайджане.

Молекулярные маркеры SSR-23.03, Rvi18-SSR, T6, NZmsCN943818 и NH030a генов устойчивости к парше Rvi12, Rvi18, Rvi11, Rvi16, а также CH03c02 гена устойчивости к мучнистой росе Pl-d не были обнаружены ни в одном из исследуемых местных сортов. 30 молекулярных маркеров, использованных для тестирования генов устойчивости к V. inaequalis и P. leucotricha, могут быть использованы для интрогрессии и пирамидизации генов устойчивости в национальной селекционной программе яблони в Азербайджане.

Об авторе

Э. М. Ханкишиева
Научно-исследовательский институт плодоводства и чаеводства Министерства сельского хозяйства, Абшеронская опытная станция
Азербайджан

ул. Али Исазаде, 28, 1045, Баку, Азербайджан



Список литературы

1. Kaymak, S. Apple scab disease caused by Venturia inaequalis [(Cooke) G. Winter1875] Turkey isolates determination of molecular characterization and pathogenicity : Ph.D Thesis. – Konya, 2012. – 122 p.

2. Jafarov, İ. Agricultural Phytopathology / İ. Jafarov. – Baku : Science publishing house, 2001. – 277 p.

3. Janick, J. Fruit breeding / ed. J. Janick, J. N. Moore // New York, USA : Tree and Tropical Fruits. – 1996. – Vol. 1. – 77 p.

4. Williams, E. B. A new physiologic race of Venturia inaequalis, incitant of apple scab / E. B. Williams, A. G. Brown // Plant Disease Reporter. – 1968. – 52 (10). – P. 799–801.

5. Williams, E. Resistance in Malus to Venturia inaequis / E. Williams, J. Kuc // Annual Review of Phytopathology. – 1969. – 7. – P. 223–246.

6. Molecular markers linked to the apple scab resistance gene Vbj derived from Malus baccata jackii / M. Gygax [et al.] // Journal of Theoretical and Applied Genetics. – 2004. – 109(8). – P. 1702–1709. doi: 10.1007/s00122-004-1803-9.

7. A new race of Venturia inaequalis virulent to apples with resistance due to the Vf gene / L. Parisi [et al.] // Phytopathology. – 1993. – 83(5). – P. 533–537.

8. Schorfresistente Sorten: Nach wie vor ein wichtiger Baustein zur nachhaltigen Obstproduktion / A. Peil [et al.] // Obstbau. – 2014. – 39. – S. 131.

9. Breeding elite lines of apple carrying pyramided homozygous resistance genes against apple scab and resistance against powdery mildew and fire blight / I. O. Baumgartner [et al.] // Plant Molecular Biology Reporter. – 2015. – 33 (5). – P. 1573–1583. doi:10.1007/s11105-015-0858-x.

10. Resistance breeding in apple at Dresden-Pillnitz / A. Peil [et al.] // In: Weinsberg FÖOEV, ed. Proceedings of the 13th International Conference on Cultivation Technique and Phytopathological Problems in Organic Fruit-Growing. – 2008. – 220 (5). – P. 220–225.

11. Development and test of 21 multiplex PCRs composed of SSRs spanning most of the apple genome / A. Patocchi [et al.] // Tree Genetics & Genomes. – 2009. – 5. – P. 211–223.

12. Identification by genome scanning approach (GSA) of a microsatellite tightly associated with the apple scab resistance gene Vm / A. Patocchi [et al.] // Tree Genetics & Genomes. – 2005. – 48(4). – P. 630–636.

13. The Vh2 and Vh4 scab resistance genes in two differential hosts derived from Russian apple R12740-7A map to the same linkage group of apple / V.G.M. Bus [et al.] // Molecular Breeding. – 2005b. – 15 (1). – P. 103-116. doi: 10.1007/s11032-004-3609-5.

14. Revision of nomenclature of the differential host-pathogen interactions of Venturia inaequalis and Malus / V. G. M. Bus [et al.] // Annual Review of Phytopathology. – 2011. – 49. – P. 391–413. doi: 10.1146/annurev-phyto-072910-095339.

15. Aligning male and female linkage maps of apple (Malus pumila Mill.) using multi-allelic markers / C. Maliepaard [et al.] // TAG Theoretical and Applied Genetics. – 1998. – 97 (1). – P. 60–73.

16. Identification and mapping of markers for resistance to apple scab from ‘Antonovka’ and ‘Hansen’s baccata#2’ / M. Hemmat [et al.] // Journal of Acta Horticulturae. – 2003. – 622. – P. 153–161. doi: 10.17660/ActaHortic.2003.622.13.

17. Mapping of the apple scab-resistance gene Vb / N. Erdin [et al.] // Tree Genetics & Genomes. – 2006. – 49 (10). – P. 1238–1245.

18. Characterisation and genetic mapping of a major scab resistance gene from the old Italian apple cultivar ‘Durello di Forli’ / S. Tartarini [et al.] // Acta Horticulturae. – 2004. – 663. – P. 129–134.

19. Inheritance studies of apple scab resistance and identification of Rvi14, a new major gene that acts together with other broad-spectrum QTL / V. Soufflet-Freslon [et al.] // Tree Genetics & Genomes. – 2008. – 51 (8). – P. 657–667.

20. Vr(2): a new apple scab resistance gene / A. Patocchi [et al.] // Theoretical and Applied Genetics. – 2004. – 109 (5). – P. 1087–1092.

21. Fine mapping of the gene Rvi18 (V25) for broad-spectrum resistance to apple scab, and development of a linked SSR marker suitable for marker-assisted breeding / J. M. Soriano [et al.] // Molecular breeding. – 2014. – 34 (4). – P. 2021–2032.

22. High-resolution genetic and physical map of the Rvi1 (Vg) apple scab resistance locus / V. Cova [et al.] // Molecular Breeding. – 2015. – 35 (16). – P. 1–13. doi: 10.1007/s11032-015-0245-1.

23. The Vh8 locus of a new gene-for-gene interaction between Venturia inaequalis and the wild apple Malus sieversii is closely linked to the Vh2 locus in Malus pumila R12740-7A / V. G. M. Bus [et al.] // New Phytologist. – 2005a. – 166 (3). – P. 1035–1049. doi: 10.1111/j.1469-8137.2005.01395.x.

24. Isolation of two microsatellite markers from BAC clones of the Vf scab resistance region and molecular characterisation of scab resistant accessions in Malus germplasm / B. A. Vinatzer [et al.] // Journal of Plant Breeding. – 2004. – 123. – P. 312–326.

25. Fine-mapping of the apple scab resistance locus Rvi12 (Vb) derived from ‘Hansen’s baccata#2’ / S. Padmarasu [et al.] // Mol Breeding. – 2014. – 34. – P. 2119–2129. doi: 10.1007/s11032-014-0167-3.

26. Simple sequence repeats for the genetic analysis of apple / L. Gianfranceschi [et al.] // Journal of Plant Pathology. – 1998. – 96. – P. 1069–1076.

27. Genome mapping of an apple scab, a powdery mildew and a woolly apple aphid resistance gene from open-pollinated Mildew Immune Selection / V. G. M. Bus [et al.] // Tree Genetics & Genomes. – 2010. – 6. – P. 470–477. doi:10.1007/s11295-009-0265-2.

28. Construction of a dense genetic linkage map for apple rootstocks using SSRs developed from Malus ESTs and Pyrus genomic sequences / J. M. Celton [et al.] // In Tree Genetics & Genomes. – 2009. – 5 (1). – P. 93–107. doi: 10.1007/s11295-008-0171-z.

29. Genetic linkage maps constructed by using an interspecific cross between Japanese and European pears / T. Yamamoto [et al.] // Theoretical and Applied Genetics. – 2002. – 106. – P. 9–18.

30. Identification of PCR-based markers linked to the powdery-mildew resistance gene Pl1 from Malus robusta in cultivated apple / T. Markussen [et al.] // Plant Breeding. – 1995. – 114. – P. 530–534. doi.org/10.1111/j.1439-0523.1995.tb00850.x.

31. Efficient low-cost DNA extraction and multiplex fluorescent PCR method formarker-assisted selection in breeding / J. E. Frey [et al.] // Plant Breeding. – 2004. – 123. – P. 554–557.

32. Candidate resistance genes from an EST database prove a rich source of markers for major genes conferring resistance to important apple pests and diseases / S. E. Gardiner [et al.] // Acta Horticulturae. – 2003. – 622. – P. 141–151.

33. James, C. M. Identification of molecular markers linked to the mildew resistance genes Pl-d and Pl-w in apple / C. M. James, K. M. Evans // Acta Horticulturae. – 2004. – 663. – P. 123–127. doi.org/10.1007/s00122-004-1836-0.

34. Seglias, N. Genetics of apple powdery mildew resistance from Malus zumi (Pl2) / N. Seglias, C. Gessler // IOBC (WPRS) Bulletin: Integrated control of pome fruit diseases. – 1997. – 20. – P. 195–208.

35. Venturia inaequalis Resistance in Apple / C. Gessler [et al.] // Critical Reviews in Plant Sciences. – 2006. – 25. – P. 473–503.

36. Patzak, J. Identification of Apple Scab and Powdery Mildew Resistance Genes in Czech Apple (Malus × domestica) Genetic Resources by PCR Molecular Markers / J. Patzak, F. Paprštein, A. Henychová // Czech J. Genet. Plant Breed. – 2011. – 47 (4). – P. 156–165.

37. Localisation of a major gene for apple scab resistance on the European genetic map of the Prima × Fiesta cross / C. Durel [et al.] // IOBC wprs Bulletin. – 2000. – 23(12). – P. 245–248.


Рецензия

Для цитирования:


Ханкишиева Э.М. Скрининг устойчивости местных сортов яблони Азербайджана к патогенам Venturia inaequalis (Cooke.) Wint. и Podosphaera leucotricha Salm. с использованием молекулярных маркеров. Плодоводство. 2020;32(1):44-52.

For citation:


Khankishiyeva E.M. Screening for resistance of local Azerbaijan apple varieties to Venturia inaequalis (Cooke.) Wint. and Podosphaera leucotricha Salm. pathogen using molecular markers. Fruit Growing. 2020;32(1):44-52. (In Russ.)

Просмотров: 81


Creative Commons License
Контент доступен под лицензией Creative Commons Attribution 4.0 License.


ISSN 0134-9759 (Print)