Study of the genetic diversity of raspberry samples using SSR markers
https://doi.org/10.1234/0134-9759-2023-35-54-53
Abstract
The current level of breeding development and the introduction of new technologies require enhanced oversight over the quality and authenticity of breeding achievements. Against this background development of molecular and genetic passports of plant varieties as an original genetic information repository that proves the authenticity of a breeding product has become a high priority issue.
Since 2019 the RUE “Institute of Fruit Growing” has been working on development of a molecular and genetic passports database for collection samples of raspberry varieties of domestic and foreign breeding with summer fruiting period that are necessary to oversee the process of adding new samples to the collection to avoid duplication, as well as to establish intraspecific interactions and species relatedness of genotypes using SSR analysis, which significantly extends the capability of variety verification.
Eight microsatellite SSR markers were used to create the passports. As a result, a high level of polymorphism of 8 microsatellite loci was shown in 47 varieties and hybrids of raspberry of different geographical origin and 101 alleles were identified. Depending on the locus, the number of alleles varied from 7 to 19. The RhM043 locus was the most polymorphic, and the RhM001 locus was the least polymorphic.
Using the data of SSR analysis, genetic passports were compiled for 47 samples of summer-fruiting raspberry (40 varieties, 7 hybrids), among which 9 are of Belarusian selection, 24 are Russian, 4 are Ukrainian, 2 are Kazakh, 4 are Romanian, 1 are Polish and 3 are of English selection. Among the genotypes assessed, 3 samples are distinguished by the yellow color of the berries. Based on the results of the research, a dendrogram was made illustrating the genetic relationship between the genotypes of summer-fruiting raspberry.
About the Authors
T. A. Gashenko
РУП «Институт плодоводства»
Belarus
Belarus
L. V. Frolova
РУП «Институт плодоводства»
Belarus
Belarus
T. N. Bozhidai
РУП «Институт плодоводства»
Belarus
Belarus
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Review
For citations:
Gashenko T.A., Frolova L.V., Bozhidai T.N. Study of the genetic diversity of raspberry samples using SSR markers. Fruit Growing. 2023;35(2):54-61. (In Russ.) https://doi.org/10.1234/0134-9759-2023-35-54-53
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