Phytosanitary monitoring of mother and collection plantations of grape varieties and hybrids
Abstract
The grape plantations of the RUE ‘Institute of Fruit Growing’ were monitored for the presence of major and quarantine sap-transmissible viruses. 23 varieties were tested in the Department of Biotechnology and 26 varieties and hybrids of Belarusian selection were assessed in the Fruit Plants Breeding Department. In the grape collection plantations, the most commonly identified virus was GFkV, present in 23.5 % of all plants tested, including that of the Bianca, Regent, Crystall, Krasotka varieties and hybrids 2-15, 56-8. Diagnosis of plants during their dormancy period with the use of real-time PCR confirmed the presence of GFkV both in the phloem and in the leaf petiole of the grape plant.
The exceptions during real-time PCR-testing were the hybrid 52-18, in which GFkV was detected in the phloem only up to the 40th cycle (Cq – 38.93), and in the tissues of the leaf petiole the virus was not identified at all, and the Jupiter (Аs / Ас – 22.9), in which the virus was not identified in the phloem, but was identified in the petiole (Cq – 34.08). In tissue samples of the Platovsky and Bianca varieties, when diagnosed using real-time PCR, viral particles were identified in all tissues examined at cycle 30 and 40, depending on the variety, while according to the ELISA test, the virus was absent (Аs / Ас – 1.23 and 0.98 respectively).
Repeated ELISA testing of virus-free mother plants with a closed root system, kept under greenhouse conditions, during the period of active growth, confirmed the virus-free status.
About the Authors
T. A. Krasinskaya
РУП «Институт плодоводства»; Белорусский государственный университет; 3УО «Международный государственный экологический институт имени А. Д. Сахарова» Белорусского государственного университета
Belarus
Belarus
T. N. Bozhidai
РУП «Институт плодоводства»
Belarus
Belarus
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Review
For citations:
Krasinskaya T.A., Bozhidai T.N. Phytosanitary monitoring of mother and collection plantations of grape varieties and hybrids. Fruit Growing. 2024;36:80-90. (In Russ.)
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