Application of molecular genetic analysis for identification of self-incompatibility alleles in sweet cherry cultivars in Belarus
Abstract
The study of self-incompatibility in sweet cherry is a relevant scientific issue with significant practical importance, primarily due to the necessity of selecting optimal pollinizer cultivars and designing effective orchard planting schemes. Therefore, the main objective of this research was the molecular-genetic identification of self-incompatibility gene (S-gene) alleles in zoned sweet cherry cultivars. To achieve this, consensus primer pairs PaConsI and PaConsII, as well as allele-specific DNA markers, were used for additional confirmation. The S-gene allele composition was determined for 14 zoned sweet cherry cultivars, with seven alleles identified out of the 33 known. For the cultivars Belitsa, Ovstuzhenka, Severnaya, Gronkovaya, Medunitsa, and Tyutchevka, only partial allele composition was established, suggesting the presence of rare alleles. Allele frequencies were assessed, revealing that S3, S6, and S5 are the most common among the studied cultivars. Cultivars with fully identified S-gene alleles were grouped into self-incompatibility groups, resulting in eight distinct groups. The largest was Group VII (alleles S3S5), represented by five cultivars.
The data obtained on the S-gene allele composition and the grouping of cultivars by incompatibility types will be used in breeding programs and for planning commercial sweet cherry orchards.
About the Authors
T. A. Gashenko
РУП «Институт плодоводства»
Belarus
Belarus
I. G. Polubyatko
РУП «Институт плодоводства»
Belarus
Belarus
Yu. G. Kondratenok
РУП «Институт плодоводства»
Belarus
Belarus
A. A. Taranov
РУП «Институт плодоводства»
Belarus
Belarus
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Review
For citations:
Gashenko T.A., Polubyatko I.G., Kondratenok Yu.G., Taranov A.A. Application of molecular genetic analysis for identification of self-incompatibility alleles in sweet cherry cultivars in Belarus. Fruit Growing. 2025;37:82-88. (In Russ.)
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