Genetic diversity study of common cherry cultivars using SSR markers
Abstract
Microsatellite DNA markers (SSRs) are effectively used for studying the genetic diversity of germplasm and for DNA fingerprinting of fruit crop cultivars. In this study, ten SSR markers were applied to analyze polymorphism across 12 common cherry genotypes. The results indicate a relatively high level of genetic diversity among the studied cultivars. The number of alleles per locus ranged from 2 to 20, with a total of 93 alleles identified. The average number of alleles per locus across the 12 cultivars was 9.3. Among the SSR markers used, EMPA015 showed the highest allelic diversity with 20 alleles, while EMPA006 was the least polymorphic. The remaining markers revealed between 4 and 13 alleles per locus. Some cherry cultivars were found to possess rare and unique alleles. Based on the SSR data, molecular genetic profiles were constructed for the 12 cherry cultivars included in the State Register of Plant Varieties approved for cultivation in various types of farms in the Republic of Belarus.
About the Authors
I. G. Polubyatko
РУП «Институт плодоводства»
Belarus
Belarus
T. A. Gashenko
РУП «Институт плодоводства»
Belarus
Belarus
A. A. Taranov
РУП «Институт плодоводства»
Belarus
Belarus
References
1. Куликов, И. М. Значение генетических коллекций плодовых культур для инновационного развития отрасли / И. М. Куликов, Л. А. Марченко // Вестник Российской академии наук. – 2015. – № 85 (1). – С. 18.
2. Развитие научной школы по садоводству профессора В. В. Кичины, выдающегося генетика и селекционера / И. М. Куликов, Н. Г. Морозова, В. С. Симонов, О. А. Сорокопудова // Плодоводство и ягодоводство России. – 2017. – № 49. – С. 186–195.
3. AC/GT and AG/CT microsatellites repeats in peach (Prunus persica (L.) Batsch): isolation, characterization and crossspecies amplifications in Prunus / G. Cipriani, G. Lot, W.-G. Huang [et al.] // Theoretical and Applied Genetics. – 1999. – Vol. 99. – P. 65–72.
4. Development and variation analysis of microsatellite markers in peach / M. J. Aranzana, J. Garcia-Mas, J. Carbo, P. Arùs // Plant Breeding. – 2002. – Vol. 121. – P. 87–92.
5. Development of microsatellite markers in peach [Prunus persica (L.) Batsch] and their use in genetic diversity analysis in peach and sweet cherry (Prunus avium L.) / E. Dirlewanger, P. Cosson, M. Tavaud [et al.] // Theoretical and Applied Genetics. – 2002. – Vol. 105. – P. 127–138.
6. Downey, S. Polymorphic DNA markers in black cherry (Prunus serotina) are identified using sequences from sweet cherry, peach and sour cherry / S. Downey, A. Iezzoni // Journal of the American Society of Horticultural Science. – 2000. – Vol. 125. – P. 76–80.
7. DNA fingerprinting of tetraploid cherry germplasm using simple sequence repeats / C. Cantini, A. F. Iezzoni, W. F. Lamboy [et al.] // Journal of American Society of Horticultural Sciences. – 2001. – Vol. 126 (2). – P. 205–209.
8. Wünsch, A. Molecular characterisation of sweet cherry (Prunus avium L.) genotypes using peach [Prunus persica (L.) Batsch] SSR sequences / A. Wünsch, J. I. Hormaza // Heredity. – 2002. – Vol. 89. – P. 56–63.
9. Wünsch, A. Molecular evaluation of genetic diversity and S allele composition of local Spanish sweet cherry (Prunus avium L.) cultivars / A. Wünsch, J. I. Hormaza // Genetic Resources and Crop Evolution. – 2004. – Vol. 51. – P. 635–641.
10. Characterization of microsatellites in wild and sweet cherry (Prunus avium L.) – markers for individual identification and reproductive processes / S. Schueler, A. Tusch, M. Schuster, B. Ziegenhangen // Genome. – 2003. – Vol. 46 (1). – P. 95–102.
11. Vaughan, S. P. Characterization of novel microsatellites and development of multiplex PCR for large-scale population studies in wild cherry, Prunus avium / S. P. Vaughan, K. Russell // Molecular Ecology Notes. – 2004. – Vol. 4. – P. 429–431.
12. Касar, Y. A. Sweet cherry cultivar identification by using SSR markers / Y. A. Касar, A. Iezzoni, S. Cetiner // Journal of Biological Sciences. – 2005. – Vol. 5 (5). – P. 616–619.
13. Genetic variation in flowering cherries (Prunus subgenus Cerasus) characterized by SSR markers / S. Ohta, T. Katsuki, T. Tanaka [et al.] // Breeding Science. – 2005. – Vol. 55. – P. 415–424.
14. Höltken, A. M. Detecting local establishment strategies of wild cherry (Prunus avium L.) / A. M. Höltken, H. R. Gregorius // BMC Ecology. – 2006. – Vol. 6 (13).
15. Analysis of sweet cherry (Prunus avium L.) cultivars using SSR and AFLP markers / D. Struss, R. Ahmad, S. M. Southwick, M. Boritzki // Journal of the American Society for Horticultural Science. – 2003. – Vol. 128. – P. 904–909.
16. Molecular characterization of the national collection of Swiss cherry cultivars / A. Frei, D. Szalatnay, T. Zollinger, J. E. Frey // The Journal of Horticultural Science and Biotechnology. – 2010. – Vol. 85 (4). – P. 277–282.
17. Разнообразие SSR-аллелей сортов вишни обыкновенной (Prunus cerasus) / О. Ю. Урбанович, П. В. Кузмицкая, З. А. Козловская, А. А. Таранов // Весцi нацыянальнай акадэмii навук Беларусi. Серыя бiялагiчных навук. – 2014. – № 2. – С. 64–69.
18. Clarke, J. B. Development and characterization of polymorphic microsatellites from Prunus avium ‘Napoleon’ / J. B. Clarke, K. R. Tobbutt // Molecular Ecology Notes. – 2003. – Vol. 3. – № 4 – P. 578–580.
Review
For citations:
Polubyatko I.G., Gashenko T.A., Taranov A.A. Genetic diversity study of common cherry cultivars using SSR markers. Fruit Growing. 2025;37:66-72. (In Russ.)
Views:
14
Email this article 