The possibility of detection of Raspberry bushy dwarf virus (RBDV) in microshoots of raspberry plants in culture in vitro by DAS-ELISA
Abstract
It was established that Raspberry bushy dwarf virus (RBDV) can be detected in in vitro-derived shoots of raspberry by DAS-ELISA test (without a false negative results). RBDV was detected in one clone of raspberry cv. Heritage (out of 14 plants 5 clones) both in the in vitro and adaptation stages (at 1.5 and 5.5 months after planted in non-sterile conditions). In addition, seventeen clones (out of other 54 clones) of raspberry plants (in vitro shoots) were found infected with RBDV. This made it possible to exclude virus-infected clones of raspberry plants from the micropropagation process. The optical density of samples with RBDV exceeded the optical density of the negative control by 18–28 times.
About the Authors
E. V. Kolbanova
Республиканское унитарное предприятие «Институт плодоводства»
Belarus
Belarus
T. N. Bozhiday
Республиканское унитарное предприятие «Институт плодоводства»
Belarus
Belarus
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Review
For citations:
Kolbanova E.V., Bozhiday T.N. The possibility of detection of Raspberry bushy dwarf virus (RBDV) in microshoots of raspberry plants in culture in vitro by DAS-ELISA. Fruit Growing. 2018;30(1):131-135. (In Russ.)
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