RAPD-analysis of genetic stability of in vitro-propagated blueberry cv. Northblue
Abstract
Monitoring of the genetic stability among in vitro-propagated plants is of outmost importance using tissue culture as continuous source of planting material for industrial utilization. The aim of the research was to assess the genetic stability of in vitro-propagated blueberry plants (cv. Northblue). As a result of the research there was evaluated the possibility of using 54 oligonucleotide primers for analysis of the genetic stability of blueberry plants (cv. Northblue) using RAPD-PCR. 6 primers (OPA 01, OPA 10, OPE 3, OPN 14, OL 1, OL 8) which produced the maximum number of distinct stripes were selected. It was found that all RAPD profiles of micropropagated blueberry plants generated with those 6 primers were monomorphic and identical to those of the mother plant.
About the Authors
T. N. Bazhydai
РУП «Институт плодоводства»
Belarus
Belarus
N. N. Volosevich
РУП «Институт плодоводства»
Belarus
Belarus
N. V. Kuhkarchik
РУП «Институт плодоводства»
Belarus
Belarus
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Review
For citations:
Bazhydai T.N., Volosevich N.N., Kuhkarchik N.V. RAPD-analysis of genetic stability of in vitro-propagated blueberry cv. Northblue. Fruit Growing. 2015;27:180-185. (In Russ.)
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