Comparative analysis of the use of ELISA and PCR testing for diagnostics of ACLSV and ASPV in tissues of pome fruit crops during the vegetation growing season
Abstract
The research was carried out in the Biotechnology Department of the RUE ‘Institute of Fruit Growing’ in 2021–2023. The objects of the study were apple stem pitting virus (ASPV) and apple chlorotic leaf spot virus (ACLSV), along with apple trees of Papirovka Belsad, Champion, Redcraft, Aksamit, Belana, Antonovka common varieties, pear trees of Bere Alexander Luka and Conference varieties, reliably infected with the studied viruses. During different periods of the vegetation growing season, the presence or absence of viruses in leaves, buds, shoots, flowers, fruits, and seeds was assessed by ELISA and PCR (two modifications). It has been established that ACLSV and ASPV are present in varying amounts in plant organs during the vegetation period; their detection depends on the diagnostic methods used. The lack of stable results when diagnosing apple trees for ASPV using the PCR method with primers SPs635/SPas721 and the TaqMan probe SPp662 makes it impossible to recommend it for identifying plants affected by the virus.
About the Authors
N. V. Kukharchik
РУП «Институт плодоводства»
Belarus
Belarus
E. V. Kolbanova
РУП «Институт плодоводства»
Belarus
Belarus
T. N. Bozhidai
РУП «Институт плодоводства»
Belarus
Belarus
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Review
For citations:
Kukharchik N.V., Kolbanova E.V., Bozhidai T.N. Comparative analysis of the use of ELISA and PCR testing for diagnostics of ACLSV and ASPV in tissues of pome fruit crops during the vegetation growing season. Fruit Growing. 2024;36:26-31. (In Russ.)
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