Polymorphism of microsatellite loci of primocane raspberry as a basis for genetic certification of varieties

Abstract

Microsatellite DNA markers are currently effectively used in studying the genetic diversity of berry crops and DNA certification of varieties.

Using the data of SSR analysis, molecular genetic passports were compiled for 48 samples of primocane raspberry (44 varieties, 4 hybrids), 7 of which are of Belarusian selection, 21 – Russian, 5 – Ukrainian, 5 – Polish, 4 – Dutch, 3 – Swiss, 1 – Romanian, 1 – English and 1 – American. Among the genotypes assessed, 5 samples are distinguished by the yellow color of the berries. 8 microsatellite SSR markers were used to create the passports. As a result, a high level of polymorphism of these microsatellite loci was shown and 113 alleles were identified. Depending on the locus, the number of alleles varied from 6 to 26. The RhM043 locus turned out to be the most polymorphic, and the RhM001 locus turned out to be the least polymorphic.

According to the results of research, the article presents a catalog of 13 genetic passports of primocane raspberry varieties included in the State Register of Agricultural Plant Varieties and being tested by the State Inspection for Testing and Protection of Plant Varieties of the Republic of Belarus.