In vitro culture initiation of grape explants at active growth period

Abstract

Grape plant genotype was observed to determine explant regenerative in vitro capacity with a high degree of confidence when studying grape cultivar explants of cross-species hybridization (‘Regent’, ‘Mars’, ‘Bianca’, ‘Marquette’, ‘Agat donskoi’) using biologically active compounds. ‘Regent’ cultivar had the high regenerative capacity (48.6 %) by the mean of a direct organogenesis at the selected conditions.

30 % hydrogen peroxide is effective to produce sterile culture with exposition for 10 minutes. It should be take into account explants type at sterilization: apical parts of green cuts and lateral shoot are more sensitive than buds without cover scale.

1.1 mg/L 6-BА only as a biologically active compound in a modified MS medium helps to increase normally developed explants output by the mean of a direct organogenesis. Meanwhile, 0.09 mg/L NAA stimulates callusogenesis at the e[plant base by 13.4 % which is 19 times higher as for the medium with no auxin.