In vitro culture stabilization of sour cherry explants

Abstract

The dependence of explant regeneration and gemmagenesis in vitro on genotype was noted using sour cherry cultuvars Rovesnitsa, L’ubskaya, Livenskaya, Volochaevka, Griot Seridko, Zaranka, Zhivitsa and Üjfehértói Fürtös. 50 % hydrogen peroxide at the exposition of 10 min for bud sterilization at the beginning of active plant growth (the end of February - early March) is effective for getting of aseptic explants. The nutrition medium for initiation in vitro culture with IBA (0.2 mg/l) and 6-BA (1 mg/l) is optimal for explant regeneration for cv. Zaranka, Zhivitsa and Griot Seridko.

The influence of sour cherry genotype was noted on gemmagenesis. The minimum of the multiplication rate was observed on the modified Murashige-Skooga medium with 1 mg/l of 6-BA for cv. Griot Seridko and Zaranka. The combined use of cytokinin (6-BA) and auxin (NAA) improved the gemmagenesis of the cv. Zhyvitsa and Zaranka, as a result of which the multiplication rate almost doubled.