In vitro rhyzogenesis of microplants of blue honeysuckle cultivars (Lonicera caerulea L. var. kamtschatica)

Abstract

Genotype (p < 0.001), concentration of IBA in the nutrient medium (p < 0.001), and two factors together (p < 0.01) have a significant effect on the number of microplants rooted in in vitro culture, on average height and number of roots of blue honeysuckle plants. The average root length was significantly affected (p < 0.001) by the concentration of IBA and two factors together (IBA and genotype). For cv. ‘Pavlovskaya’ and cv. ‘Krupnoplodnaya’ at in vitro rhyzogenesis stage, the use of ½ macro- and microsalt MS medium with the addition of IBA at concentration of 1.5 mg/l makes it possible to obtain 78–79 % of rooted microplants (average root number – 3.96–3.24, average root length – 3.23–3.30 cm). For cv. ‘Goluboye vereteno’, the rooting rate of 93 % (average root number – 3.89, average root length – 2.78 cm) was achieved even at concentration of 1 mg/l IBA. For the cv. ‘Volkhova’, it is advisable to use IBA in a high concentration (3.0 mg/l), at which the rooting of plants increases 1.8–2.0 times compared with low concentrations of IBA 0.8–1.0 mg/l. The rooting of microplants in hormone-free medium was 17–23 %, depending on cultivar.