Relative analysis of diagnostic devices resolution for enzyme immunodetection of Plum pox virus
Abstract
The researches were executed within the limits of the contract within Belarusian Republican Fund of Fundamental Researches Nr. B13MLD-006 «Mobilization of modern bioprocessing methods for disease control of highly hazardous plum virus Sharka (Plum pox virus) in the Institute for Fruit Growing (NAS of Belarus) and in Scientific-Practical Institute of Horticulture and Food Technology (Republic Moldova). The work was done within the limits of the project „Mobilizarea mijloacelor biotehnologice moderne în lupta contra virusului periculos a vărsatului prunului (Plum pox)‟, funded by Academy of Sciences of Moldova with the project Nr. 13.820.14.06/BA. The purpose of the investigation was to study the sensitivity of the sets of instruments for diagnosis, prepared in laboratory of immunology and virology (Moldova), for enzyme immunodetection of Sharka virus in comparison with its commercial analogue. All diagnosticums such as PPV (Bioreba), C Nr. 43 (Moldova) and M Nr. 38 (Moldova) have allowed accurately revealing Sharka plum virus in samples. Excess of samples absorbency of over negative control made 4-17 times. Commercial antibodies (Bioreba) and the antibodies received on the base of isolate D (M Nr. 8 Moldova) were characterised by similar resolution, excess of samples absorbency over negative control made 1.2-4.4 and 1.3-3.4 respectively. At the same time, the highest resolution was typical for the antibodies got on the base of isolate C Nr. 43 (Moldova).
About the Authors
N. V. Kukharchik
РУП «Институт плодоводства»
Belarus
Belarus
E. V. Kolbanova
РУП «Институт плодоводства»
Belarus
Belarus
N. N. Volosevich
РУП «Институт плодоводства»
Belarus
Belarus
M. S. Kastritskaya
РУП «Институт плодоводства»
Belarus
Belarus
J. A. Kalashyan
Публичное учреждение «Научно-практический институт садоводства, виноградарства и пищевых технологий»
Moldova, Republic of
Moldova, Republic of
E. Z. Zemchik
Публичное учреждение «Научно-практический институт садоводства, виноградарства и пищевых технологий»
Moldova, Republic of
Moldova, Republic of
A. M. Chernets
Публичное учреждение «Научно-практический институт садоводства, виноградарства и пищевых технологий»
Moldova, Republic of
Moldova, Republic of
L. N. Prodanyuk
Публичное учреждение «Научно-практический институт садоводства, виноградарства и пищевых технологий»
Moldova, Republic of
Moldova, Republic of
V. I. Lukitsa
Публичное учреждение «Научно-практический институт садоводства, виноградарства и пищевых технологий»
Moldova, Republic of
Moldova, Republic of
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Review
For citations:
Kukharchik N.V., Kolbanova E.V., Volosevich N.N., Kastritskaya M.S., Kalashyan J.A., Zemchik E.Z., Chernets A.M., Prodanyuk L.N., Lukitsa V.I. Relative analysis of diagnostic devices resolution for enzyme immunodetection of Plum pox virus. Fruit Growing. 2015;27:109-114. (In Russ.)
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