RAPD-analysis of genetic stability of in vitro-propagated blueberry cv. Northblue

T. N. Bazhydai; N. N. Volosevich; N. V. Kuhkarchik

RAPD-analysis of genetic stability of in vitro-propagated blueberry cv. Northblue

T. N. Bazhydai, N. N. Volosevich, N. V. Kuhkarchik

Full Text:

PDF (Rus) |

Generate QR code

Abstract

Monitoring of the genetic stability among in vitro-propagated plants is of outmost importance using tissue culture as continuous source of planting material for industrial utilization. The aim of the research was to assess the genetic stability of in vitro-propagated blueberry plants (cv. Northblue). As a result of the research there was evaluated the possibility of using 54 oligonucleotide primers for analysis of the genetic stability of blueberry plants (cv. Northblue) using RAPD-PCR. 6 primers (OPA 01, OPA 10, OPE 3, OPN 14, OL 1, OL 8) which produced the maximum number of distinct stripes were selected. It was found that all RAPD profiles of micropropagated blueberry plants generated with those 6 primers were monomorphic and identical to those of the mother plant.

Keywords

blueberry, in vitro culture, genetic stability, RAPD-PCR, Belarus

References

1. Debnath, S.C. Propagation of Vaccinium in vitro: a review / S.C. Debnath // International Journal of Fruit Science. - 2006. - Vol. 6, № 2. - P. 47-71.

2. Debnath, S.C. Propagation and cultivation of Vaccinium species and less known small fruits / S.C. Debnath // Vaccinium ssp. and less known small fruit: challenges and risks: international scientific conference, Jelgava, 6-8 October 2009 / Latvia University of Agriculture; ed. A. Adamovics [et al.]. - Jelgava, 2009. - P. 22-29.

3. Rani, V. Genetic fidelity of organized meristem-derived micropropagated plants: a critical reappraisal / V. Rani, S.N. Raina // In Vitro Cellular and Developmental Biology - Plant. - 2000. - Vol. 36, N 5. - P. 319-330.

4. Vysotskii, V.A. O geneticheskoi stabil'nosti pri klonal'nom mikrorazmnozhenii plodovykh i yagodnykh kul'tur / V.A. Vysotskii // Sel'skokhozyaistvennaya biologiya. - 1995. - № 5. - S. 57-63.

5. Genetic instability of sugarcane plants derived from meristem cultures / M.I. Zucchi [et al.] // Genetics and Molecular Biology. - 2002. - Vol. 25. - P. 91-96.

6. Assessment of genetic stability and fidelity of some micropropagated Vitis vinifera L. «Feteasca neagra» clones by ampelometric and RAPD markers / R.N. Gheorghe [et al.] // Bulletin UASVM Horticulture. - 2009. - Vol. 66, N 1. - P. 51-57.

7. Determination of genetic stability of micropropagated plants of ginger using random amplified polymorphic DNA (RAPD) markers / G.R. Rout [et al.] // Botanical Bulletin of Academia Sinica. - 1998. - Vol. 39. - P. 23-27.

8. Evaluation of the genetic fidelity of in vitro-propagated gerbera (Gerbera jamesonii Bolus) using DNA-based markers / R. Bhatia [et al.] // Plant Cell, Tissue and Organ Culture. - 2011. - Vol. 104, N 1. - P. 131-135.

9. Martins, M. Genetic stability of micropropagated almond plantlets, as assessed by RAPD and ISSR markers reappraisal / M. Martins, D. Sarmento, M.M. Oliveira // Plant Cell Reports. - 2004. - Vol. 23, N 7. - P. 492-496.

10. Microclonal propagation of Vaccinium sp. and Rubus sp. and detection of genetic variability in culture in vitro / A. Gajdosova [et al.] // Journal of Fruit and Ornamental Plant Research. - 2006. - Vol. 14. - P. 103-119.

11. Molecular analysis of genetic stability in micropropagated apple rootstock MM106 / M. Modgil [et al.] // Scientia Horticulturae. - 2004. - Vol. 104. - P. 151-160.

12. Venkatachalam, L. Genetic analysis of micropropagated and regenerated plantlets of banana as assessed by RAPD and ISSR markers / L. Venkatachalam, R.V. Sreedhar, N. Bhagyalakshmi // In Vitro Cellular and Developmental Biology - Plant. - 2007. - Vol. 43, N 3. - P. 267-274.

13. Gosudarstvennyi reestr sortov / otv. red.: V.A. Beinya. - Minsk, 2014. - S. 83-84, 152-153.

14. Pavlovskii, N.N. Kul'tury netraditsionnogo plodovodstva v kollektsiyakh Tsentral'nogo botanicheskogo sada NAN Belarusi / N.N. Pavlovskii, T.V. Kurlovich // Tsentral'nyi botanicheskii sad NAN Belarusi: sokhranenie, izuchenie i ispol'zovanie bioraznoobraziya mirovoi flory; pod red. V.V. Titka, V.N. Reshetnikova. - Minsk, 2012. - Gl. 7. - S. 146-162.

15. Bozhidai, T.N. Vliyanie tipa eksplanta na regeneratsionnuyu sposobnost' cherniki, golubiki, brusniki i klyukvy na etape vvedeniya v kul'turu in vitro / T.N. Bozhidai, N.V. Kukharchik // Plodovodstvo: nauch. tr. / RUP «In-t plodovodstva«; redkol.: V.A. Samus' (gl. red.) [i dr.]. - Samokhvalovichi, 2014. - T. 26. - S. 241-247.

16. Bozhidai, T.N. Osobennosti razmnozheniya in vitro i ukoreneniya ex vitro golubiki sorta Northblue / T.N. Bozhidai, N.V. Kukharchik // Vestsi NAN Belarusi. Ser. biyal. navuk. - 2014. - № 4. - S. 28-31.

17. Debnath, S.C. An assessment of the genetic diversity within a collection of wild cranberry (Vaccinium macrocarpon Ait.) clones with RAPD-PCR / S.C. Debnath // Genetic Resources and Crop Evolution. - 2007. - Vol. 54. - P. 509-517.

18. Pikunova, A.V. Otsenka geneticheskogo raznoobraziya iskhodnogo i selektsionnogo materiala yagodnykh kul'tur s pomoshch'yu molekulyarnykh markerov: dis. … kand. biol. nauk: 06.01.05, 03.02.07 / A.V. Pikunova. - Orel, 2011. - 153 l.

For citations:

Bazhydai T.N., Volosevich N.N., Kuhkarchik N.V. RAPD-analysis of genetic stability of in vitro-propagated blueberry cv. Northblue. Fruit Growing. 2015;27:180-185. (In Russ.)

This work is licensed under a Creative Commons Attribution 4.0 License.

ISSN 0134-9759 (Print)

Fruit Growing

RAPD-analysis of genetic stability of in vitro-propagated blueberry cv. Northblue

Full Text:

Abstract

Keywords

About the Authors

References

Review

For citations:

Username
Password
	Remember me

User

Fruit Growing

RAPD-analysis of genetic stability of in vitro-propagated blueberry cv. Northblue

Full Text:

Abstract

Keywords

About the Authors

References

Review

For citations:

Cookies policy