Biological indexing using herbaceous indicator plants of apple mosaic virus isolates
Abstract
Apple mosaic virus (ApMV) is one of the major pathogens infecting apple trees. Chenopodium quinoa, Cucumis sativus, Phaseolus vulgaris and Catharantus roseus are often used as herbaceous indicator plants for multiplication of the virus in plant tissues and for the virus detection. It is difficult to transmit ApMV onto herbaceous plants due to an extreme instability of the virus. The aim of the research was to estimate the influence of buffer concentration and stabilizers on the transmission of the virus from apple leaves to C. quinoa, as well as to estimate the possibility of ApMV transmission from C. quinoa to indicator plants of C. sativus, P. vulgaris и C. roseus. The comparison of four inoculation buffers for transmission of the virus onto C. quinoa indicator plants showed that there was no difference on their affectivity. The results of SC-RT-PCR confirmed the transmission of the virus from C. quinoa to C. sativus и P. vulgaris plants in spite of the lack of specific symptoms of the infection.
About the Authors
N. N. Volosevich
РУП «Институт плодоводства»
Belarus
Belarus
N. V. Kukharchik
РУП «Институт плодоводства»
Belarus
Belarus
M. Cieslinska
Научно-исследовательский институт садоводства
Poland
Poland
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Review
For citations:
Volosevich N.N., Kukharchik N.V., Cieslinska M. Biological indexing using herbaceous indicator plants of apple mosaic virus isolates. Fruit Growing. 2014;26(1):79-84. (In Russ.)
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