Diagnosis efficiency of Belarusin isolates of Plum pox virus in laboratory
Abstract
The investigations were made within 2011-2012 in the department of biotechnology of the Institute for Fruit Growing. Preliminary visual diagnosis of plum pox virus made at the plantations of stone fruit crops such as plum, cherry plum, sour cherry and sweet cherry ones during their vegetation, i.e. in the end of spring and in the beginning of summer. Diagnosis efficiency of Belarusian isolates of plum pox virus by means of a number of laboratory methods, including use of polyclonal, recombinant and monoclonal universal antibodies for enzyme immunoassay and IC-RT-PCR method was estimated.
It is shown, that polyclonal antibodies are more effective for mass diagnosis of Belarusian isolates of plum pox virus, than monoclonal universal antibodies.
At the same time the variant of enzyme immunoassay with use of polyclonal antibodies is the least sensitive. The maximum dilution, at which it has been possible to define the presence of the given virus in plant tissues, does not exceed 1:20. The use of monoclonal and recombinant antibodies in the enzyme immunoassay allowed defining the virus in the samples diluted up to 1:100 and at the IC-RT-PCR method it became possible at dilution of extracts up to 1:50000.
About the Authors
N. V. Kukharchik
РУП «Институт плодоводства»
Belarus
Belarus
M. S. Kastritskaya
РУП «Институт плодоводства»
Belarus
Belarus
E. V. Kolbanova
РУП «Институт плодоводства»
Belarus
Belarus
O. V. Solovej
РУП «Институт плодоводства»
Belarus
Belarus
N. V. Volosevich
РУП «Институт плодоводства»
Belarus
Belarus
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Review
For citations:
Kukharchik N.V., Kastritskaya M.S., Kolbanova E.V., Solovej O.V., Volosevich N.V. Diagnosis efficiency of Belarusin isolates of Plum pox virus in laboratory. Fruit Growing. 2014;26(1):128-135. (In Russ.)
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