Analysis of infection rate by Plum pox virus of plum, cherry plum and sour cherry cultivars in Belarus

Abstract

The analysis of infection rate of plum, cherry plum, sour cherry and sweet cherry cultivars of the Belarusian assortment by Sharka plum virus (Plum pox virus) was carried out. Preliminary investigations were made by the method of routing inspections. Basic researches were carried out with the help of enzyme immunoassay using polyclonal antibodies (РАb) and universal monoclonal ones (PPV-UnMAbs) to the plum pox virus, as well as with the help of IC-RT-PCR method with primers PPV-P1/PPV-P2. Monitoring of plum pox virus in mother and cutting plantations, in mother plantations of clonal stocks and in industrial orchards was made within 2000-2013, which allowed estimating the infection rate of certain cultivars and stock forms by the virus on a natural infectious background (59 genotypes).

Plum pox virus has been revealed at numerous fruit crops cultivars. Among them are plum cultivars Nagrada nemanskaya, Vitebskaya pozdnyaya, Empress and Prezident; cherry plum ones such as Kometa, Naidzena and Vetraz‘; cherry cultivar Vyanok; sweet cherry one Iputs; stocks OD-2-3, VVA-1, 140-2, L-2 and seed stocks. The greatest number of plants affected by Plum pox virus was at the plum cultivar Nagrada nemanskaya and cherry plum cultivar Kometa.